Ortho Tolidine Test For Blood Results Color Chart Orthotolidine may be incorporated into a reagent strip such as the Multistix Ames A negative result is yellow A positive result shows a green or blue color The reaction should be read within 10 seconds It is a sensitive test detecting blood a high dilution
Positive Test Result An immediate pink color is seen Negative Test No color change is seen Sensitivity The sensitivity of the phenolphthalein test can be discussed with the dilution factor of blood samples Yellow color is produced and matched against charts within 10 sec this gives the amount of free chlorine The yellow color appearing after a few minutes gives the amount of Total chlorine
Ortho Tolidine Test For Blood Results Color Chart
Ortho Tolidine Test For Blood Results Color Chart
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Add 10 to 15 drops of orthotolidine to the test sample mix and let stand for 5 minutes Keep the sample in the dark during the 5 minute color development to reduce false color caused by manganese and nitrate compounds Add on Add on Stability in days d or hours hrs Test Name Alt Spec Add on Submit specimens on ice Carboxyhemoglobin NaHep 0 5hr Blood Culture Quantitative None Protect specimens from light Electrolytes Whole Blood None 0 5hr Pour Plate submit along with blood culture bottles Glucose Whole Blood NaHep 0 5hr Flow Cytometry
If you re a phlebotomist curious about the tests you re drawing for or a patient wondering about your blood test this article is here to help We ll explain what the different colors mean and the tests associated with each The orthotolidine test however is not accurate because the impurities such as iron manganese nitrate etc are likely to cause a false yellow color and indicating wrong and increased chlorine residual
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Match color in center test cell test tube with a color standard The Slide is in proper alignment for a color match when a white line on the Slide is directly above the white line on the comparator base Record as parts per million ppm total chlorine Cl2 Orthotolidine o tolidine can be used to help identify a blood stain
TEST FOR DETECTING BLOOD IN URINE 1 Enumerate the tests for detection of blood in urine 2 What is the chemical test for occult blood 3 What is the principle of Benzidine test Hemoglobin has peroxidase activity which releases nascent oxygen from H2O2 Nascent oxygen oxidizes Benzidine in acid solution to form blue dye 4 Development of color Standard glucose solutions were taken in 6 test tubes in the range of 0 2 to 1 mL 1 mL of protein free filtrate was taken in a seventh test tube To all these tubes 5 mL of o toluidine was added and mixed thoroughly The concentration of blood glucose in a given sample is mg mL
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Orthotolidine may be incorporated into a reagent strip such as the Multistix Ames A negative result is yellow A positive result shows a green or blue color The reaction should be read within 10 seconds It is a sensitive test detecting blood a high dilution
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Positive Test Result An immediate pink color is seen Negative Test No color change is seen Sensitivity The sensitivity of the phenolphthalein test can be discussed with the dilution factor of blood samples
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Ortho Tolidine Test For Blood Results Color Chart - Tetramethylbenzidine and the orthotolidine tests were apt to produce interference color reactions with plant peroxidases A maximum of 10 seconds for color development is required for reliable sensitivity whereas in high blood concentrations the color change will