Clinical Microbiology Bacteria Flow Chart Blood Agar Macconkey Oxidase Catalase

Clinical Microbiology Bacteria Flow Chart Blood Agar Macconkey Oxidase Catalase In this section there are six flowcharts and three tables to help you organize the different types of bacteria media and tests and a few other tips for remembering commonly seen pathogens and tests

MacConkey Agar Lactose Fermentation on MAC Enterobacteriaceae HACEK AACEK and other GN group of bacteria has not been properly expounded upon Please do additional readings Oxidase Acinetobacter Stenotrophomonas Proteus Yersinia Salmonella Shigella B mallei Numerous tests and bacteria have been omitted PP MESSY Motility PEMPSY Gram Negative Cocci Obligate anaerobic Veillonella spp Aerobic Facultative Anaerobic M catarrhalis Neisseria spp Bacilli Obligate anaerobic Bacteroides spp

Clinical Microbiology Bacteria Flow Chart Blood Agar Macconkey Oxidase Catalase

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If the bacteria contain catalase the mixture bubbles as the hydrogen peroxide decomposes into water and oxygen In the clinic the catalase test helps distinguish catalase positive Staphylococci from catalase negative Streptococcus which are both Gram positive cocci H influenzae and H haemolyticus will grow in the hemolytic zone of S aureus on blood agar plates the hemolysis of cells by S aureus releases factor V which is needed for its growth H influenza and H haemolyticus will not grow outside the hemolytic zone of S aureus due to the lack of nutrients such as V factor in these areas

This paper reviewed core concepts of interpreting bacterial culture results including timing of cultures common culture sites potential for contamination interpreting the Gram stain role of rapid diagnostic tests conventional antibiotic susceptibility testing and automated testing There are a lot of different types of agar plates used in the microbiology lab but the three most common are sheep blood agar chocolate and MacConkey MacConkey agar is selective for G bacilli and further differentiates G bacilli through lactose fermentation On MacConkey agar lactose fermenting bacteria will turn a bright pink color

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agar plates On agar media containing blood it exhibits glistening haemolytic colonies and develops an average diameter of 2 0mm in 1 to 2 days Colonies equally grow well on MacConkey agar They are oxidase positive and motile by peritrichous flagella They are also nitrate and urease positive usually within 4hr which is a MacConkey agar MAC is a bacterial culture medium named after bacteriologist Alfred T MacConkey 1861 1931 MacConkey agar is a selective and differentiating agar that only grows gram negative bacterial species it can further differentiate the gram negative organisms based on their lactose metabolism 1 The selective and

Catalase neutralizes the bactericidal effects of hydrogen peroxide 13 and its concentration in bacteria has been correlated with pathogenicity 8 Enzyme based tests play a crucial part in the identification of bacteria Clinical significance They are the cause of invasive neonatal infections Neonatal infections present as two different clinical entities early onset neonatal disease characterized by sepsis and pneumonia within the first 7 days of life late onset disease with meningitis and sepsis between 7 days and 3 month of age

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Microbiology Clinical Laboratory Science Resource

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In this section there are six flowcharts and three tables to help you organize the different types of bacteria media and tests and a few other tips for remembering commonly seen pathogens and tests

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MacConkey Agar Lactose Fermentation on MAC Enterobacteriaceae HACEK AACEK and other GN group of bacteria has not been properly expounded upon Please do additional readings Oxidase Acinetobacter Stenotrophomonas Proteus Yersinia Salmonella Shigella B mallei Numerous tests and bacteria have been omitted PP MESSY Motility PEMPSY


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Clinical Microbiology Bacteria Flow Chart Blood Agar Macconkey Oxidase Catalase - H influenzae and H haemolyticus will grow in the hemolytic zone of S aureus on blood agar plates the hemolysis of cells by S aureus releases factor V which is needed for its growth H influenza and H haemolyticus will not grow outside the hemolytic zone of S aureus due to the lack of nutrients such as V factor in these areas